Development of a Cryo-Raman Microscope for High-Sensitivity Observation of Molecules in Frozen Biological Samples
December 13, 2024
The new method produces images that are up to eight times brighter than previous Raman microscopy images
A joint research group from Osaka University, Kyoto Prefectural University of Medicine, and the RIKEN CSRS has developed a "Cryo-Raman Microscope" that improves the signal-to-noise ratio (SNR) by rapidly freezing biological samples and performing Raman observations at a constant low temperature.
Raman scattering signals are inherently weak, making it challenging to achieve a high SNR in Raman observations of biological samples. Strong laser light or prolonged exposure can damage the samples, and sample movement can further complicate the process. Additionally, conventional fixation methods often alter the chemical state of the samples, posing another limitation.
The Cryo-Raman Microscope addresses these challenges by minimizing laser-induced damage and preserving the molecular distribution and chemical state of the samples. Using this microscope, the research group successfully conducted long-term observations of frozen cells, confirming enhanced observation signals, improved SNR, and increased spatial and spectral resolution.
The new technique can be combined with other microscopy techniques for detailed analysis of biological samples and is expected to contribute to a wide range of areas in the biological sciences including medicine and pharmaceutics.
- Original article
- Science Advances doi: 10.1126/sciadv.adn0110
- K. Mizushima, Y. Kumamoto, S. Tamura, M. Yamanaka, K. Mochizuki, M. Li, S. Egoshi, K. Dodo, Y. Harada, N. I. Smith, M. Sodeoka, H. Tanaka, K. Fujita,
- "Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imagingRaman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging".
- Contact
- Mikiko Sodeoka; Group Director
Kosuke Dodo; Senior Research Scientist
Syusuke Egoshi; Visiting Scientist
Catalysis and Integrated Research Group
